Voltammetric Evaluation of the Binding between Wheat Germ Agglutinin and Thionine/Glucose-modified Magnetic Microbeads
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چکیده
منابع مشابه
Voltammetric evaluation of the binding between wheat germ agglutinin and thionine/glucose-modified magnetic microbeads.
The binding between glucose residues and wheat germ agglutinin (WGA) on thionine/glucose-modified magnetic microbeads was evaluated using voltammetry. Thionine is an electroactive compound and has two amino groups. Thionine was immobilized to magnetic beads via cross-linking of the amino groups on the beads with an amino group on thionine. Glucose was bound to the other amino group of thionine ...
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Procedures for the isolation, purification, and crystallization of wheat germ agglutinin are described. The agglutinin was purified 184-fold to homogeneity from commercial wheat germ lipase. A molecular weight of 23,500 was estimated for the protein by means of sedimentation equilibrium and sodium dodecyl sulfate gel electrophoresis. The agghxtinin is a glycoprotein. Amino acid and carbohydrate...
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In this study, we revealed that the lectin wheat germ agglutinin (WGA) and its specific binding sugars, N-acetylneuraminic acid (NeuAc) and Nacetylglucosamine (GlcNAc), inhibited mating pair formation in Paramecium caudatum. The concentrations that caused 50% inhibition (IC50) were 17 nM, 3 mM and 30 mM, respectively. Using FITCWGA, it was shown fluorescence-cytochemically that WGA bound to the...
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The inhibition of carbohydrate-protein interactions by tailored multivalent ligands is a powerful strategy for the treatment of many human diseases. Crucial for the success of this approach is an understanding of the molecular mechanisms as to how a binding enhancement of a multivalent ligand is achieved. We have synthesized a series of multivalent N-acetylglucosamine (GlcNAc) derivatives and s...
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Wheat germ agglutinin (WGA) stimulated neutrophils to produce significant levels of luminol-dependent chemiluminescence (CL). Since WGA is known to bind N-acetylglucosamine (GlcNAc) oligomers and N-acetylneuraminic acid (NANA), we attempted to determine which binding property of WGA is essential for induction of CL. The succinylated form of WGA (SuWGA), which is no longer able to bind NANA, was...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2008
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.24.717